Detection of E. Coli and Coliform Bacteria
VIT® E. coli/Coliforme clearly detects all coliform germs and, in parallel, bacteria of the E. coli species in one analysis. Even lactose- and indole-negative E. coli strains are reliably detected. The reliability and specificity of this detection distinguishes it from all other methods available on the market.
The detection is easy to carry out - it only takes a few minutes. The results of the analysis are available after just 3 hours. The analysis is carried out using a fluorescence microscope. All essential consumables for carrying out the analysis are included in the kit. Package size: 25 analyses.
Your Advantages
VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.
The VIT® gene probes hybridize only with intact, metabolically active cells, as only these have sufficient amounts of ribosomal RNA. This means that only living Cronobacter are detected, while dead or inactive cells are excluded.
Complete detection is completed in just 3-5 hours - a decisive advantage over cultivation-based methods, which take several days due to enrichment, incubation and evaluation. The VIT® technology therefore enables significantly faster decisions in quality assurance, process control and release.
The test kits are user-friendly and can be integrated into existing laboratory procedures with minimal training. Detection is carried out using fluorescence microscopy and does not require complex equipment or special software solutions. This makes the method particularly robust and user-independent.
The VIT® technology is based on over 25 years of experience in the development of specific, fluorescence-labeled gene probes for microbiological analysis. It is characterized by high specificity, reproducibility and resistance to interfering matrices.
A particular advantage: as the method is based directly on rRNA and does not require enzymatic amplification steps, it is insensitive to inhibitors. False-positive results, which can occur with PCR-based test kits - especially in complex matrices - are therefore ruled out.
Product Specifications
| Detection of | coliform germs |
| Samples that can be examined include | Isolates (direct) Liquid enrichment (direct) Water (short pre-enrichment) Food (short pre-enrichment) Beverages (short pre-enrichment) |
| Technology | VIT® gene probe technology |
| Evaluation | by means of VIT® adapted Fluorescence microscope |
| Type of analysis | qualitative detection |
| Scope of delivery | Analysis reagents Product manual |
| Package size | 25 analyses |
Handling and Analysis
The sample is prepared using a VIT® adapted fluorescence miscroscope. All bacteria are visible in phase contrast. Coliform cells glow red and E. coli specifically green.
This product is also available as a service:
Detection of Escherichia coli / coliform bacteria
If you want an absolute quantification of the bacteria present in the sample in addition to reliable identification, we recommend:
You Might Also Be Interested in
Detection of Microorganisms in Sausage, Meat and Fish
In food processing companies, it is necessary to test for various pathogenic microorganisms such as salmonella, listeria, staphylococci and others in the final product inspection. Highly specific detection is mandatory, as contaminated products pose…
Milk and Dairy Products
Milk and dairy products are home to a variety of microorganisms. In many cases, the bacteria are desired or even necessary. In this context, starter cultures, which are used for the targeted ripening of cheese or sour cream butter, for example, are…
Starter Cultures
Microbial starter cultures are used for the targeted microbial manipulation of a product. Starter cultures can be used in various areas of food production, such as yoghurt, cheese and sour milk products. Starter cultures can be present both in pure…