L/D Analysis for the Quality Assurance of Probiotics
Probiotic products contain live microorganisms that support intestinal health. However, the effectiveness of these products depends directly on the number of live bacteria. With the live/dead analysis (L/D analysis), we offer a reliable, fast and precise method to monitor and guarantee the viability of the bacteria.
Your Advantages
The L/D analysis uses flow cytometry in combination with two fluorescent dyes that reliably distinguish live and dead cells based on membrane integrity. The method provides an objective and reproducible assessment of cell viability, as each cell is individually and automatically quantified.
Due to the high measurement speed of flow cytometry, results are available within a very short time. The fully automated workflow enables fast sample throughput and supports efficient decision-making in quality control and batch release.
The method detects even the smallest changes in cell membrane integrity and reliably identifies low proportions of viable or damaged cells. Through single-cell analysis via flow cytometry, a high-resolution viability profile is generated that remains accurate even at low cell concentrations.
The flow cytometry–based L/D analysis is suitable for various probiotic product formats and can be applied at all stages of product development and quality control. As it operates independently of cultivation, it provides a reliable assessment of viability even in complex product matrices.
Why Is the L/D Analysis Important?
Quality Assurance
The L/D analysis makes it possible to differentiate between dead and living bacteria, which are active in probiotic products and offer health benefits.
Stability Monitoring
The L/D analysis makes it possible to check the vitality of the bacteria during storage to ensure that the products maintain their shelf life and effectiveness.
Consumer Confidence
Probiotic products must have minimum levels of live bacteria. The L/D analysis helps manufacturers to meet the highest standards and gain the trust of consumers.
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