Detection of Beer Spoilage Organisms
VIT® Leuconostoc/Lactococcus lactis enables the rapid and specific identification of live Leuconostoc spp. on the one hand and Lactococcus lactis bacteria on the other. They are displayed in different colors. In the beverage sector, both in-process and end product samples are analyzed for the presence of these bacteria. With VIT® Leuconostoc spp./Lactococcus lactis, only live bacteria are detected - so you only find microorganisms that are actually relevant.
The kit is the ideal complement to VIT® Beer plus L. brevis (the same enrichments can be used) and VIT® Beer Megasphaera/Pectinatus.
The detection is easy to carry out - it only takes a few minutes. The results of the analysis are available after just 3 hours. The analysis is carried out using a fluorescence microscope. All essential consumables for carrying out the analysis are included in the kit. Package size: 25 analyses.
Your Advantages
VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.
The VIT® gene probes hybridize only with intact, metabolically active cells, as only these have sufficient amounts of ribosomal RNA. This means that only living Leuconostoc spp. and Lactococcus lactis are detected, while dead or inactive cells are excluded.
Complete detection is completed in just 3-5 hours - a decisive advantage over cultivation-based methods, which take several days due to enrichment, incubation and evaluation. The VIT® technology therefore enables significantly faster decisions in quality assurance, process control and release.
The test kits are user-friendly and can be integrated into existing laboratory procedures with minimal training. Detection is carried out using fluorescence microscopy and does not require complex equipment or special software solutions. This makes the method particularly robust and user-independent.
The VIT® technology is based on over 25 years of experience in the development of specific, fluorescence-labeled gene probes for microbiological analysis. It is characterized by high specificity, reproducibility and resistance to interfering matrices.
A particular advantage: as the method is based directly on rRNA and does not require enzymatic amplification steps, it is insensitive to inhibitors. False-positive results, which can occur with PCR-based test kits - especially in complex matrices - are therefore ruled out.
Product Specifications
| Detection of | Leuconostoc spp. and Lactococcus lactis |
| Samples that can be analyzed include | Isolates (direct) Liquid enrichment (direct) Beer and beer-based mixed drinks (short pre-enrichment) Hygiene samples (short pre-enrichment) |
| Technology | VIT® gene probe technology |
| Evaluation | by means of VIT® adapted Fluorescence microscope |
| Type of analysis | qualitative detection |
| Scope of delivery | Analysis reagents Product manual |
| Package size | 25 analyses |
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