Detection of E. Coli and Coliform Bacteria
VIT® E. coli/Coliforme clearly detects all coliform germs and, in parallel, bacteria of the E. coli species in one analysis. Even lactose- and indole-negative E. coli strains are reliably detected. The reliability and specificity of this detection distinguishes it from all other methods available on the market.
Coliform bacteria are used as a hygiene criterion in water analysis, E. coli specifically as an indicator of fecal contamination. With VIT® E. coli/Coliforme, a targeted and rapid assessment of microbiological water quality is possible.
Your Advantages
VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.
The VIT® gene probes only hybridize with intact, metabolically active cells, as only these have sufficient quantities of ribosomal RNA. This means that only living coliforms and Escherichia coli are detected, while dead or inactive cells are excluded.
Complete detection is completed in just 3-5 hours - a decisive advantage over cultivation-based methods, which take several days due to enrichment, incubation and evaluation. The VIT® technology therefore enables significantly faster decisions in quality assurance, process control and release.
The test kits are user-friendly and can be integrated into existing laboratory procedures with minimal training. Detection is carried out using fluorescence microscopy and does not require complex equipment or special software solutions. This makes the method particularly robust and user-independent.
The VIT® technology is based on over 25 years of experience in the development of specific, fluorescence-labeled gene probes for microbiological analysis. It is characterized by high specificity, reproducibility and resistance to interfering matrices.
A particular advantage: as the method is based directly on rRNA and does not require enzymatic amplification steps, it is insensitive to inhibitors. False-positive results, which can occur with PCR-based test kits - especially in complex matrices - are therefore ruled out.
Product Specifications
| Detection of | coliform germs and specifically of Escherichia coli |
| Samples that can be analyzed include | Isolates (direct) Liquid enrichment (direct) Water (short pre-enrichment) Food (short pre-enrichment) Beverages (short pre-enrichment) |
| Technology | VIT® gene probe technology |
| Evaluation | by means of VIT® adapted Fluorescence microscope |
| Type of analysis | qualitative detection |
| Scope of delivery | Analysis reagents Product manual |
| Package size | 25 analyses |
Handling and Analysis
The sample is prepared using a VIT® adapted fluorescence miscroscope. All bacteria are visible in phase contrast. Coliform cells glow red and E. coli specifically green.
You can also have your sample analyzed in-house:
Laboratory analysis E. coli/coliforms
If, in addition to reliable identification, you would like an absolute quantification of the bacteria present in the sample, we recommend:
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