Laboratory Analysis: Detection of Lactic Acid Bacteria and L. Brevis
All beer spoiling lactic acid bacteria and, in parallel, the most common beer spoiler Lactobacillus brevis are clearly detected. All important stages in the brewing process can be analyzed: Filtrate and non-filtrate area incl. yeast recirculation.
You receive the results on the 3rd working day after sample receipt. In the case of finished enrichments, at the latest on the 1st working day after sample receipt.
Qualitative or quantitative detection?
Qualitative
How is the analysis carried out?
By using the reliable VIT® gene probe technology.
What are the requirements for the sample to be sent in?
Product or raw material samples:
- Beer, wine, juices & soft drinks, min. 100 ml
- Water samples (e.g. mineral water, rinse water etc.), min. 100 ml
Pre-enriched samples:
- Enrichment broth (10 ml) or agar plate with colonies: This leads to an acceleration of the detection.
Ideal for analysis directly on site:
Your Advantages
VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.
The VIT® gene probes hybridize only with intact, metabolically active cells, as only these have sufficient amounts of ribosomal RNA. This means that only living beer spoiling lactic acid bacteria L. brevis are detected, while dead or inactive cells are excluded.
Complete detection is completed in just 3-5 hours - a decisive advantage over cultivation-based methods, which take several days due to enrichment, incubation and evaluation. The VIT® technology therefore enables significantly faster decisions in quality assurance, process control and release.
The VIT® technology is based on over 25 years of experience in the development of specific, fluorescence-labeled gene probes for microbiological analysis. It is characterized by high specificity, reproducibility and resistance to interfering matrices.
A particular advantage: as the method is based directly on rRNA and does not require enzymatic amplification steps, it is insensitive to inhibitors. False-positive results, which can occur with PCR-based test kits - especially in complex matrices - are therefore ruled out.
You Might Also Be Interested in
VIT® Beer Screening
Unwanted microorganisms in beer? This test kit specifically identifies all beer spoilage organisms from the genera Lactobacillus and Pediococcus.
VIT® technology
The fast, reliable and highly specific VIT® gene probe technology is the further development and industrial standard of FISH technology for the detection of microorganisms.
Beer and Mixed Drinks
Microbiological quality control of raw materials and products in the brewing industry. Fast, specific and reliable - no inhibitors, no false-negative / false-positive results.