Detection of Pathogens: Pseudomonas Aeruginosa
Pseudomonas aeruginosa is a gram-negative bacterium that is widespread in the environment and is feared as a pathogenic microorganism or pathogen. The detection of this bacterium is of great importance in many areas of application, but is often very difficult to accomplish. With Scan VIT® Pseudomonas aeruginosa, a test is available that makes the analysis of bathing water in particular child's play and does not require many years of laboratory experience. After filtration of the test sample and brief cultivation, the test confirms all micro-colonies that have grown, not just a selection as in the classic method. The results are highly specific and are available at an impressive speed. The analysis is completed after 27 hours. Only living bacteria are detected.
The detection is easy to carry out - it only takes a few minutes. The analysis is carried out using a fluorescence microscope. All essential consumables for carrying out the analysis are included in the kit.
For non-filterable materials, we recommend our test kit:
You can also have your sample analyzed in-house:
Quantification of Pseudomonas aeruginosa
Your Advantages
VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.
The VIT® gene probes hybridize only with intact, metabolically active cells, as only these have sufficient amounts of ribosomal RNA. This means that only living Pseudomonas aeruginosa are detected, while dead or inactive cells are excluded.
Complete detection is completed in just 3-5 hours - a decisive advantage over cultivation-based methods, which take several days due to enrichment, incubation and evaluation. The VIT® technology therefore enables significantly faster decisions in quality assurance, process control and release.
The test kits are user-friendly and can be integrated into existing laboratory procedures with minimal training. Detection is carried out using fluorescence microscopy and does not require complex equipment or special software solutions. This makes the method particularly robust and user-independent.
The VIT® technology is based on over 25 years of experience in the development of specific, fluorescence-labeled gene probes for microbiological analysis. It is characterized by high specificity, reproducibility and resistance to interfering matrices.
A particular advantage: as the method is based directly on rRNA and does not require enzymatic amplification steps, it is insensitive to inhibitors. False-positive results, which can occur with PCR-based test kits - especially in complex matrices - are therefore ruled out.
Product Specifications
| Detection of | Pseudomonas aeruginosa |
| Samples that can be analyzed include | Bathing water Drinking water filterable materials |
| Technology | Scan VIT® technology |
| Evaluation | by means of VIT® adapted Fluorescence microscope |
| Type of analysis | quantitative detection |
| Scope of delivery | Analysis reagents Product manual |
| Package size | 20 or 100 analyzes |
| Limit of detection | 1 CFU / 100 ml |
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