Laboratory Analysis: Detection of E. Coli and Coliform Bacteria
Your sample is analyzed for the presence of coliform bacteria and Escherichia coli .
You will receive the results on the 3rd working day after receipt of the sample; in the case of pre-enriched samples or grown colonies, the results will be available on the 1st working day after receipt of the sample at the latest.
Qualitative or quantitative detection?
Qualitative
How is the analysis carried out?
By using the reliable VIT® gene probe technology.
What are the requirements for the sample to be sent in?
Product or raw material samples:
- Beer, wine, juices & soft drinks, min. 100 ml
- Juice concentrates, min. 25 ml
- Powder, min. 10 g
- Water samples (e.g. mineral water, rinse water etc.), min. 150 ml
Pre-enriched samples:
- Enrichment broth (10 ml) or agar plate with colonies: This leads to an acceleration of the analysis.
Our test kit is recommended for analyzing your samples directly on site:
Your Advantages
VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.
The VIT® gene probes only hybridize with intact, metabolically active cells, as only these have sufficient quantities of ribosomal RNA. This means that only living Escherichia coli and coliform bacteria are detected, while dead or inactive cells are excluded.
Complete detection is completed in just 3-5 hours - a decisive advantage over cultivation-based methods, which take several days due to enrichment, incubation and evaluation. The VIT® technology therefore enables significantly faster decisions in quality assurance, process control and release.
The VIT® technology is based on over 25 years of experience in the development of specific, fluorescence-labeled gene probes for microbiological analysis. It is characterized by high specificity, reproducibility and resistance to interfering matrices.
A particular advantage: as the method is based directly on rRNA and does not require enzymatic amplification steps, it is insensitive to inhibitors. False-positive results, which can occur with PCR-based test kits - especially in complex matrices - are therefore ruled out.
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