Detection of Clinically Relevant Antibiotic Resistance Genes in Wastewater
The qPCR VIT® AMR I Test Kit enables the specific and robust detection and quantification of the antibiotic resistance genes blaOXA-48-like, blaKPC , and blaCTX-M in treated wastewater samples using quantitative PCR (qPCR). The standardized analysis enables reliable and reproducible quantification of the selected target genes.
The blaOXA-48-like and blaKPC genes encode carbapenemases that confer resistance to carbapenems. Carbapenems are among the most important reserve antibiotics in human medicine. Genes of the CTX-M family encode extended-spectrum β-lactamases (ESBLs), which confer resistance to various β-lactam antibiotics.
Together with the qPCR Prep AMR Test Kit, the qPCR VIT® AMR I Test Kit forms a comprehensive workflow for the standardized analysis of wastewater samples - from sample preparation to the molecular detection of selected antibiotic resistance genes. The vermicon AMR workflows are also used as part of the German nationwide AMELAG project by the Robert Koch Institute (RKI) and the German Federal Environment Agency (UBA) to analyze antibiotic resistance genes in complex wastewater samples.
Your Benefits
Specially developed by experts in wastewater analysis. The optimized workflow ensures reliable detection of antibiotic resistance genes, even in complex wastewater samples with varying compositions.
Using a multiplex approach, it simultaneously detects the β-lactam resistance genes blaOXA-48-like, blaCTX-M, and blaKPC. This allows for the detection of both carbapenemase- and ESBL-associated resistance genes in a single test.
The three resistance genes and the bacterial reference marker 16S rDNA are detected and quantified in parallel using a multiplex qPCR assay. The reference marker is used to normalize the results and enables standardized and comparable analysis.
The qPCR VIT® product line is a comprehensive system for wastewater surveillance. It combines sample preparation, analysis, evaluation, and monitoring of health-related viruses and antibiotic resistance genes—to provide scientifically sound, comparable, and reliable data.
Meets all requirements under the Wastewater Directive (EU) 2024/3019.
Product Specifications
| Detection | Molecular qPCR-based detection of the β-lactam resistance genes blaOXA-48-like, blaCTX-M, and blaKPC in wastewater samples |
| Detection control | Positive and negative controls |
| Universal bacterial reference marker 16S rDNA as an in-process control during sample preparation | |
| Classification of the measured resistance gene concentrations using 16S rDNA as a marker for total bacterial load | |
| Result | Quantitative detection of antibiotic resistance genes using qPCR analysis |
| Report of results in, for example, gene copies/L using quantification standards included in the test kit | |
| Contents | All essential consumables for sample preparation included in the test kit |
| Package Size | 100 reactions per kit |
Complete System for Detecting Antibiotic Resistance in Wastewater
qPCR Prep AMR
Purification of wastewater samples for molecular biological qPCR analysis of antibiotic resistance genes
qPCR VIT® AMR II
Detection of β-lactam resistance genes:
- blaOXA-48-like,
- blaCTX-M, and
- blaKPC
qPCR VIT® AMR II
Detection of the β-lactam resistance gene and the sulfonamide resistance gene:
- blaNDM
- sul1
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