Laboratory Analysis: Detection of Alicyclobacillus
With the specific VIT® gene probes, all bacteria of the genus Alicyclobacillus and especially Alicyclobacillus acidoterrestris are clearly detected. Both parameters are identified. The spores of these bacteria can also be reliably identified. Detection takes place in fruit concentrates, fruit juices, soft drinks and other beverages.
You will receive the results on the 3rd working day after receipt of the sample, in the case of finished enrichments or grown colonies at the latest on the 1st working day after receipt of the sample.
Qualitative or quantitative detection?
Qualitative
How is the analysis carried out?
By using the reliable VIT® gene probe technology.
What are the requirements for the sample to be sent in?
Product or raw material samples of juices & soft drinks, min. 100 ml
Product or raw material samples of juice concentrates, min. 25 ml
Pre-enriched samples:
Enrichment broth (10 ml) or agar plate with colonies: This leads to an acceleration of the detection.
Would you like to analyze your samples directly on site? No problem with the test kits:
Flow VIT® Alicyclobacillus
Your Advantages
VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.
The VIT® gene probes hybridize only with intact, metabolically active cells, as only these have sufficient amounts of ribosomal RNA. This means that only living Alicyclobacillus spp. and Alicyclobacillus acidoterrestris are detected, while dead or inactive cells are excluded.
Complete detection is completed in just 3-5 hours - a decisive advantage over cultivation-based methods, which take several days due to enrichment, incubation and evaluation. The VIT® technology therefore enables significantly faster decisions in quality assurance, process control and release.
The VIT® technology is based on over 25 years of experience in the development of specific, fluorescence-labeled gene probes for microbiological analysis. It is characterized by high specificity, reproducibility and resistance to interfering matrices.
A particular advantage: as the method is based directly on rRNA and does not require enzymatic amplification steps, it is insensitive to inhibitors. False-positive results, which can occur with PCR-based test kits - especially in complex matrices - are therefore ruled out.
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