Nitrifying bacteria

Specific identification and quantification of ammonia-oxidizing
and nitrite-oxidizing bacteria in activated sludge samples


Item Number: 01130001

Lab analysis: detection of nitrifying bacteria

Identification and quantification of the ammonia-oxidizing and nitrite-oxidizing bacteria in wastewater samples by using our test kit Nitri-VIT®. Both important bacteria groups are analyzed and are tested for possible spatial interactions. The analysis is ideal for monitoring, for analysis of special events or for having a reference value in case process changes are planned. Even before nitrification problems occur and increase of ammonia in the effluent can be measured, the decline of one or both populations indicate future problems. Important hints for adjusting process parameters can be given before major problems arise.

Results are displayed according to the VIT® key. The report includes an informative photo documentation visualizing the nitrifying bacteria groups in the sample material.

Qualitative or quantitative detection?
Semi-quantitative detection

Which method is applied?
The reliable VIT® gene probe technology.

Which requirements are important?
Please request our simple protocol for fixation of wastewater samples. Make sure that you fixate/stabilize your samples in the indicated manner before sending the samples to us.


For a detailed profile of nitrifyer populations in wastewater samples, we recommend our Profiling of individual nitrifying bacteria populations.

For an on-site analysis in your laboratory, we recommend our test kit Nitri-VIT®.


Identical microscopic image: phase contrast (left), all nitrite-oxidizing bacteria shining red and all ammonia-oxidizing bacteria shining specifically green after analysis with gene probe technology (right).
Identical microscopic image after VIT® analysis: phase contrast, nitrite-oxidizing bacteria shine green, ammonia-oxidizing bacteria red.

Your Advantages

  • specific detection of ammonia and nitrite oxidizing bacteria

  • detection of living bacteria exclusively

  • quantification of organisms even in dense sludge flocs

  • applied for the prevention of disturbances