Detection of Yeasts in the Course of Microbiological Quality Control
Candida intermedia is a yeast that is harmful to beverages. In non-carbonated beverages, it can lead to odor and taste defects as well as to clarification and biofilm formation and is therefore particularly feared by fruit juice producers.
With the VIT® Candida intermedia test kit, this potentially fermentable yeast species is detected quickly and specifically. This means that the cause of certain product defects can be clearly identified and the test kit can be used to quickly and easily search for the origin of the contamination on the farm.
Your Advantages
VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.
The VIT® gene probes hybridize only with intact, metabolically active cells, as only these have sufficient amounts of ribosomal RNA. This means that only living Candida intermedia are detected, while dead or inactive cells are excluded.
Complete detection is completed in just 3-5 hours - a decisive advantage over cultivation-based methods, which take several days due to enrichment, incubation and evaluation. The VIT® technology therefore enables significantly faster decisions in quality assurance, process control and release.
The test kits are user-friendly and can be integrated into existing laboratory procedures with minimal training. Detection is carried out using fluorescence microscopy and does not require complex equipment or special software solutions. This makes the method particularly robust and user-independent.
The VIT® technology is based on over 25 years of experience in the development of specific, fluorescence-labeled gene probes for microbiological analysis. It is characterized by high specificity, reproducibility and resistance to interfering matrices.
A particular advantage: as the method is based directly on rRNA and does not require enzymatic amplification steps, it is insensitive to inhibitors. False-positive results, which can occur with PCR-based test kits - especially in complex matrices - are therefore ruled out.
Product Specifications
| Detection of | Candida intermedia |
| Samples that can be examined include | Isolates Liquid enrichment Biofilms Beverages, alcoholic and non-alcoholic Fruit juice and concentrates |
| Technology | VIT® gene probe technology |
| Evaluation | by means of VIT® adapted Fluorescence microscope |
| Type of analysis | qualitative detection |
| Scope of delivery | Analysis reagents Product manual |
| Package size | 25 analyses |
Detect Beverage Pests Quickly and Specifically
Alicyclobacillus, fermentable yeasts and other harmful germs can cause considerable economic losses. Modern quality control must ensure that such germs can be detected quickly, specifically and reliably. With vermicon's products and services, beverage pests can be identified quickly and reliably. Find out more!