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Laboratory Analysis: Detection of Pectinatus spp. and M. Cerevisiae

All bacteria of the genus Pectinatus and, in parallel, Megasphaera cerevisiae are clearly detected in one analysis. The dangerous beer spoilage bacteria are detected quickly and clearly in beer, environmental and process samples.

You receive the results on the 3rd working day after receipt of the sample. In the case of finished enrichments or biofilm samples, at the latest on the 1st working day after sample receipt.

Qualitative or quantitative detection?
Qualitative

How is the analysis carried out?
By using the reliable VIT® gene probe technology.

What are the requirements for the sample to be sent in?
Product or raw material samples:

  • Beer and beer-based mixed drinks, min. 100 ml
  • Water samples (e.g. mineral water, rinse water, etc.), min. 150 ml


Pre-enriched samples:

  • Enrichment broth (10 ml) or agar plate with colonies: This leads to an acceleration of the rapid analysis (the findings are then expected as early as the 1st working day after receipt of the sample).


Biofilm:

  • Please request our sampling protocol for biofilm samples.

 

Would you like to analyze your samples in your laboratory on site? No problem with our test kit:

VIT® Beer Megasphaera/Pectinatus

 

 

Your Advantages

VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.

The VIT® gene probes only hybridize with intact, metabolically active cells, as only these have sufficient amounts of ribosomal RNA. This means that only living beer spoilage bacteria Pectinatus and Megasphaera cerevisiae are detected, while dead or inactive cells are excluded.

Complete detection is completed in just 3-5 hours - a decisive advantage over cultivation-based methods, which take several days due to enrichment, incubation and evaluation. The VIT® technology therefore enables significantly faster decisions in quality assurance, process control and release.

The VIT® technology is based on over 25 years of experience in the development of specific, fluorescence-labeled gene probes for microbiological analysis. It is characterized by high specificity, reproducibility and resistance to interfering matrices.
A particular advantage: as the method is based directly on rRNA and does not require enzymatic amplification steps, it is insensitive to inhibitors. False-positive results, which can occur with PCR-based test kits - especially in complex matrices - are therefore ruled out.

Further Laboratory Services for the Detection of Beer Spoilage Organisms

Beer Spoilers - Screening PLUS

Rapid detection of a comprehensive selection of beer spoiling lactic acid bacteria, Pectinatus spp. and Megasphaera cerevisiae.

Beer Spoilers - Screening

Screening for a comprehensive selection of relevant harmful lactic acid bacteria in product or raw material samples.

Beer Spoilage Lactic Acid Bacteria

Qualitative, parallel detection of all beer spoiling lactic acid bacteria and Lactobacillus brevis in samples from the brewery/beverage sector.

Megasphaera/Pectinatus

Parallel detection of the genus Pectinatus and Megasphaera cerevisiae in beer and raw material or water samples.

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