Purification Kit for the qPCR VIT® AMR Assay
The qPCR Prep AMR test kit was specifically developed for the purification of wastewater samples to enable reliable and robust qPCR analysis using the qPCR VIT® AMR I and AMR II test kits. Microbial DNA is efficiently isolated and purified from complex wastewater samples, thereby laying the foundation for reproducible and meaningful qPCR results.
The qPCR Prep AMR test kit enables a standardized and efficient workflow for sample preparation in the laboratory. The optimized purification process reduces the influence of the complex wastewater matrix and provides microbial DNA for the subsequent molecular biological detection of selected antibiotic resistance genes via qPCR.
Integrating the test kit into the AMR workflow ensures consistent analytical conditions. This supports the comparability of results across different samples, measurement time points, and testing locations.
Complete System for Detecting Antibiotic Resistance Genes in Wastewater
qPCR Prep AMR
Purification of wastewater samples for molecular biological qPCR analysis of antibiotic resistance genes
qPCR VIT® AMR II
Detection of β-lactam resistance genes:
- blaOXA-48-like,
- blaCTX-M, and
- blaKPC
qPCR VIT® AMR II
Detection of the β-lactam resistance gene and the sulfonamide resistance gene:
- blaNDM
- sul1
Your Benefits
Specially developed for complex wastewater samples. The optimized workflow minimizes the impact of the variable wastewater matrix and common PCR inhibitors, thereby creating optimal conditions for reliable qPCR analysis.
It enables standardized purification of wastewater samples and the isolation of microbial DNA. As a result, the test kit provides the basis for reproducible analytical conditions for the subsequent molecular detection of selected antibiotic resistance genes.
The AMR workflows developed by vermicon AG are being used as part of the German AMELAG project for the molecular biological qPCR analysis of antibiotic resistance genes in wastewater. The findings obtained through this work are continuously incorporated into the optimization and further development of the analytical methods.
A standardized workflow reduces method-related differences between samples and measurement time points and improves the comparability of results in recurring analyses and long-term monitoring programs.
The qPCR VIT® product line is a comprehensive system for wastewater surveillance. It combines sample preparation, analysis, evaluation, and monitoring of health-related viruses and antibiotic resistance genes—to provide scientifically sound, comparable, and reliable data.
Directive (EU) 2024/3019 requires the monitoring of antimicrobial resistance in municipal wastewater. The qPCR VIT® AMR product line enables the standardized detection of selected antibiotic resistance genes and thus supports the implementation of the corresponding AMR monitoring program.
Product Specifications
| Detection | Purification of wastewater samples for molecular biological qPCR analysis of antibiotic resistance genes using the qPCR VIT® AMR test kits |
| Extraction Control | The efficiency of sample purification can be monitored using the extraction control (DNA Spike-In) included in the test kit |
| Result | Purified and isolated microbial DNA as the basis for subsequent molecular qPCR detection of selected antibiotic resistance genes |
| Contents | All essential consumables for sample preparation are included in the test kit |
| Package Size | 50 purifications per kit |
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