Detection of Beer Spoiling Lactic Acid Bacteria
VIT® Lactic Acid Bacteria detects live lactic acid bacteria of the genera Lactobacillus spp., Pediococcus spp. and Leuconostoc spp. in beverage samples. VIT® Lactic Acid Bacteria saves up to 2 days compared to conventional methods.
The detection is easy to carry out - it only takes a few minutes. The results of the analysis are available after just 3 hours. The analysis is carried out using a fluorescence microscope. All essential consumables for carrying out the analysis are included in the kit. Package size: 25 analyses.
Your Advantages
VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.
The VIT® gene probes hybridize only with intact, metabolically active cells, as only these have sufficient amounts of ribosomal RNA. Only living Lactobacillus spp., Pediococcus spp. and Leuconostoc spp. are detected, while dead or inactive cells are excluded.
Complete detection is completed in just 3-5 hours - a decisive advantage over cultivation-based methods, which take several days due to enrichment, incubation and evaluation. The VIT® technology therefore enables significantly faster decisions in quality assurance, process control and release.
The test kits are user-friendly and can be integrated into existing laboratory procedures with minimal training. Detection is carried out using fluorescence microscopy and does not require complex equipment or special software solutions. This makes the method particularly robust and user-independent.
The VIT® technology is based on over 25 years of experience in the development of specific, fluorescence-labeled gene probes for microbiological analysis. It is characterized by high specificity, reproducibility and resistance to interfering matrices.
A particular advantage: as the method is based directly on rRNA and does not require enzymatic amplification steps, it is insensitive to inhibitors. False-positive results, which can occur with PCR-based test kits - especially in complex matrices - are therefore ruled out.
Product Specifications
| Detection of | Lactobacillus spp., Pediococcus spp. and Leuconostoc spp. |
| Samples that can be analyzed include | Isolates (direct) Liquid enrichment (direct) Beverages (short pre-enrichment) Food (short pre-enrichment) |
| The technology | VIT® gene probe technology |
| Evaluation | by means of VIT® adapted Fluorescence microscope |
| Type of analysis | qualitative detection |
| Scope of delivery | Analysis reagents Product manual |
| Package size | 25 analyses |
Handling and Analysis
The sample is prepared using a VIT® adapted fluorescence miscroscope. All bacteria are visible in phase contrast. Lactic acid bacteria specifically glow red.
You can also have your sample analyzed in-house:
Laboratory analysis of lactic acid bacteria
Have your sample analyzed for all relevant beverage pests:
Detect Beverage Pests Quickly and Specifically
Alicyclobacillus, fermentable yeasts and other harmful germs can cause considerable economic losses. Modern quality control must ensure that such germs can be detected quickly, specifically and reliably. With vermicon's products and services, beverage pests can be identified quickly and reliably. Find out more!
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