Quantitative detection of E. coli / coliform bacteria
Scan VIT® E. coli/Coliforms enables reliable identification and absolute quantification of Escherichia coli and coliform bacteria. The results are highly specific and are available at an impressive speed. The absolute quantitative data is available within 12 hours. This gives you quick certainty as to whether - and if so how many - bacteria are present in the analyzed sample. Bacteria of the genus Escherichia coli and, in parallel, coliform bacteria are clearly detected in one analysis. The test confirms all microcolonies that have grown, not just a selection as in the classic method.
The test is easy to perform - it only takes a few minutes. The results of the analysis are available after just 12 hours. The analysis is carried out using a fluorescence microscope. All essential consumables for carrying out the analysis are included in the kit.
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Your advantages
VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.
The VIT® gene probes hybridize only with intact, metabolically active cells, as only these have sufficient amounts of ribosomal RNA. This means that only living Escherichia coli and coliform bacteria are detected, while dead or inactive cells are excluded.
Complete detection is completed in just 3-5 hours - a decisive advantage over cultivation-based methods, which take several days due to enrichment, incubation and evaluation. The VIT® technology therefore enables significantly faster decisions in quality assurance, process control and release.
The test kits are user-friendly and can be integrated into existing laboratory procedures with minimal training. Detection is carried out using fluorescence microscopy and does not require complex equipment or special software solutions. This makes the method particularly robust and user-independent.
The VIT® technology is based on over 25 years of experience in the development of specific, fluorescence-labeled gene probes for microbiological analysis. It is characterized by high specificity, reproducibility and resistance to interfering matrices.
A particular advantage: as the method is based directly on rRNA and does not require enzymatic amplification steps, it is insensitive to inhibitors. False-positive results, which can occur with PCR-based test kits - especially in complex matrices - are therefore ruled out.
Product specifications
| Detection of | Escherichia coli and coliform bacteria |
| Samples that can be analyzed include | Drinking, cooling, process and bathing water (short pre-enrichment) |
| The technology | Scan VIT® technology |
| Analysis | by means of VIT® adapted Fluorescence microscope |
| Type of analysis | quantitative detection |
| Scope of delivery | Analysis reagents Product manual |
| Package size | 100 analyses |
| Limit of detection | 1 CFU / 100 ml |
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