Rapid detection of beer spoilage organisms
VIT® Beer is the most widely used rapid detection system in breweries worldwide. Its specificity and reliability, combined with the ability to detect only live cells, have established VIT® Beer as a permanent fixture in many breweries.
VIT® Beer plus L. brevis detects all beer spoiling lactic acid bacteria and at the same time identifies the most common beer spoiler Lactobacillus brevis in one analysis.
VIT® Beer plus L. brevis saves four to five days compared to conventional culture methods. All important stages in the brewing process can be analyzed with VIT® Beer plus L. brevis: Filtrate and non-filtrate area incl. yeast recirculation.
The kit is the ideal complement to VIT® Beer Megasphaera/Pectinatus
The detection is easy to carry out - it only takes a few minutes. The results of the analysis are available after just 3 hours. The analysis is carried out using an inexpensive fluorescence microscope. All essential consumables for carrying out the analysis are included in the kit. Package size: 25 analyses.
Your advantages
VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.
The VIT® gene probes hybridize only with intact, metabolically active cells, as only these have sufficient quantities of ribosomal RNA. This means that only living lactic acid bacteria and Lactobacillusbrevis are detected, while dead or inactive cells are excluded.
Complete detection is completed in just 3-5 hours - a decisive advantage over cultivation-based methods, which take several days due to enrichment, incubation and evaluation. The VIT® technology therefore enables significantly faster decisions in quality assurance, process control and release.
The test kits are user-friendly and can be integrated into existing laboratory procedures with minimal training. Detection is carried out using fluorescence microscopy and does not require complex equipment or special software solutions. This makes the method particularly robust and user-independent.
The VIT® technology is based on over 25 years of experience in the development of specific, fluorescence-labeled gene probes for microbiological analysis. It is characterized by high specificity, reproducibility and resistance to interfering matrices.
A particular advantage: as the method is based directly on rRNA and does not require enzymatic amplification steps, it is insensitive to inhibitors. False-positive results, which can occur with PCR-based test kits - especially in complex matrices - are therefore ruled out.
Product specifications
| Detection of | Lactic acid bacteria and Lactobacillus brevis |
| Samples that can be analyzed include | Isolates (direct) Liquid enrichment (direct) Beer and beer-based mixed drinks (short pre-enrichment) Hygiene samples (short pre-enrichment) |
| Technology | VIT® gene probe technology |
| Evaluation | by means of VIT® adapted Fluorescence microscope |
| Type of analysis | qualitative detection |
| Scope of delivery | Analysis reagents Product manual |
| Package size | 25 analyses |
Handling and analysis
The sample is prepared using a VIT® adapted fluorescence miscroscope. All bacteria are visible in phase contrast. Live beer spoiling lactic acid bacteria glow red. Lactobacillus brevis is directly identified by its green color.
You can also have your sample analyzed in-house:
Laboratory analysis of lactic acid bacteria / L.brevis
Or you can have a complete VIT® Beer Screening carried out:
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