Beverage Pests: Detection of Alicyclobacillus
Aseptic taste characterizes samples contaminated with Alicyclobacillus spp. With VIT® Alicyclobacillus , however, contamination can be safely avoided. This is because VIT® Alicyclobacillus clearly detects all bacteria of the genus Alicyclobacillus spp. and Alicyclobacillus acidoterrestris in a single analysis.
The spores of this bacterium can also be reliably identified in all stages of fruit juice and fruit concentrate production.
The detection of Alicyclobacillus spp. and A. acidoterrestris is easy to carry out - it only takes a few minutes. The results of the analysis are available after just 3 hours. The analysis is carried out using a fluorescence microscope. All essential consumables for carrying out the analysis are included in the kit. Package size: 25 analyses.
Your Advantages
VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.
The VIT® gene probes hybridize only with intact, metabolically active cells, as only these have sufficient amounts of ribosomal RNA. This means that only living Alicyclobacillus spp. and Alicyclobacillus acidoterrestris are detected, while dead or inactive cells are excluded.
Complete detection is completed in just 3-5 hours - a decisive advantage over cultivation-based methods, which take several days due to enrichment, incubation and evaluation. The VIT® technology therefore enables significantly faster decisions in quality assurance, process control and release.
The test kits are user-friendly and can be integrated into existing laboratory procedures with minimal training. Detection is carried out using fluorescence microscopy and does not require complex equipment or special software solutions. This makes the method particularly robust and user-independent.
The VIT® technology is based on over 25 years of experience in the development of specific, fluorescence-labeled gene probes for microbiological analysis. It is characterized by high specificity, reproducibility and resistance to interfering matrices.
A particular advantage: as the method is based directly on rRNA and does not require enzymatic amplification steps, it is insensitive to inhibitors. False-positive results, which can occur with PCR-based test kits - especially in complex matrices - are therefore ruled out.
Product Specifications
| Detection of | Alicyclobacillus spp. and Alicyclobacillus acidoterrestris |
| Samples that can be analyzed include | Isolates (direct) Liquid enrichment (direct) Beverages (short pre-enrichment) Fruit juice and fruit concentrates (short pre-enrichment) |
| Technology | VIT® gene probe technology |
| Evaluation | by means of VIT® adapted Fluorescence microscope |
| Type of analysis | qualitative detection |
| Scope of delivery | Analysis reagents Product manual |
| Package size | 25 analyses |
Handling and Analysis
The sample is prepared using a VIT® adapted fluorescence miscroscope. All bacteria are visible in phase contrast. Bacteria of the genus Alicyclobacillus spp. glow green and A. acidoterrestris specifically red.
You can also have your sample analyzed in-house:
alicyclobacillus laboratory analysis
Or:
Detect Beverage Pests Quickly and Specifically
Candida intermedia, fermentable yeasts and other harmful germs can cause considerable economic losses. Modern quality control must ensure that such germs can be detected quickly, specifically and reliably. With vermicon's products and services, beverage pests can be identified quickly and reliably. Find out more!
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