Probiotics: New microbiological
methods for exceptional quality
Direct, modern microbiology with VIT®: The industrial standard for Fluorescence in Situ
Hybridization (FISH). State-of-the-art identity, purity, stability, viability and activity analysis,
thanks to rapid enumeration of multiple bacterial species directly in the sample.
Why do we need new, more efficient methods for probiotical analysis?
The development of probiotics requires profound microbiological understanding of the relationships and interactions between the selected microorganisms as well as with the host microbiome ecosystem. Further, the manufacturing and quality control of probiotics requires exact enumeration of all individual bacterial species in probiotic blends and determination of their activity status, including non-cultiviable bacteria.
However, there are certain obstacles and limitations of conventional and novel microbiological methods, like next generation sequencing (NGS) and flow cytometry, that researchers have to overcome in order to gain the required knowledge.
Why VIT® and Flow VIT®?
VIT® and Flow VIT® can specifically identify multiple bacteria populations in the same sample, distinguish between live and dead cells and are cultivation independent. The bets of both worlds - Flow VIT® combines molecular methods (fluorescence in situ hybridization) with flow cytometry and in this way enables rapid and accurate enumeration of specific bacterial species, making this technology inevitable for the quality control and efficiency of next-genereation probiotics.
Our mission is to dissolve the methodological limitations and enable the development and production of exceptional probiotics. Using VIT® makes that possible.
- Direct and rapid microbiology
- Patented FISH technologies, recognized by the European Pharmacopoeia
- GMP certified analysis methods
- Visualization directly in the sample
- Specific identification of multiple bacterial species
- Insights into the microbe-to-microbe interactions
- Flow VIT® provides accurate and reliable enumeration of multiple bacterial species directly in the probiotic product
- Reduces drastically the analysis time compared to traditional methods
- Deeper understanding of the microbe-to-microbe interactions
- Viability, activity and stability validation of multiple species
VIT® is a powerful molecular technique that utilizes specially programmed fluorescent-labeled probes to detect and identify microbial rRNA in a sample. The evaluation of the results can be performed using either fluorescence microscopy or flow cytometry (Flow VIT®).
Using the VIT® and Flow VIT® technologies, we perform a wide range of laboratory analyses under both GMP and non-GMP conditions. Additionally, our detection systems include standard and customized test kits and enable an easy to use in-house analysis of microorganisms.
With over 25 years of experience in analyzing complex microbiological ecosystems, we offer expert consulting services to help solve even the most challenging microbiological questions.
Sample types and industries
- raw materials
- solid and liquid samples
- single strain probiotic formulations
- multi-species probiotic formulations
- in vivo assays
- in vitro assays
- dietary supplements
- functional & fermented foods
- feed mills & livestock breeding
We work in trustful collaboration with our clients. Our service portfolio offers a broad range of possibilities. The standard services cover a large proportion of analyses relevant for the whole probiotics production process - from research and development to quality control of the final product. In case customized solution is needed, we are happy to consult you.
Microbiome analytics are a highly sensitive area. In order to provide our customers with maximum safety, we are certifying our services according to the GMP (Good Manufacturing Practice) and ISO9001: 2015 standards.
rapid enumeration of multiple bacterial species in the same sample
spatial analysis, identification and visualization
viability, activity and safety analysis
validation of stability and viability throughout shelf life
in vitro GI-tract simulation assay development
composition validation of end products
microbial quality monitoring
control of contaminants
If you need further assistance or have any questions related to our services and detection methods, please do not hesitate to contact our team of experts. We will be pleased to help you.
„The importance of bacteria for our health is becoming increasingly obvious. Probiotic drugs open up new avenues in the fight against civilization diseases such as allergies, respiratory diseases and gastrointestinal diseases. With this in mind, SymbioPharm GmbH is committed to consistently and continuously expanding all activities from research to the finished product, searching for new ways and finding unusual treatments.
For more than two decades, vermicon AG has been our accompanying, reliable partner in the research development and quality assurance of our products. With their innovative methods, microbiological know-how and professional way of working, we have found exactly the partner that complements us. The fast exchange with our direct contact person and the entire team in mutual trust is the guarantee for an uncomplicated and successful cooperation.“
Dr. Kurt Zimmermann, Chief Scientific Officer (CSO), SymbioPharm GmbH
We start where conventional methods and approaches fail. With our experience, gathered in more than 25 years in the analysis of biocenoses, we answer even highly complex, interdisciplinary microbiological questions.
enumeration of viable aerobic & anaerobic bacteria
pioneers in the application of VIT® (industrial standard of FISH) and Flow VIT®
highly-qualified experts with decades of experience in applied microbiology
innovative and experimental approach
customer focused and solution driven
The human microbiome is a highly complex ecosystem. Products based on living microorganisms generally don’t work in a direct way, but interact in a multifactorial mode of action.
To ensure the efficacy and safety of Live Biotherapeutics, a deeper understanding of the relationship between the microbiome and the human health is needed.
Expertise and technology
vermicon supports pharmaceutical companies and developers of microbiome-based products whenever a specific microbiological expertise, technological equipment or process optimization is needed.