Flow VIT® for Parallel and
Specific Enumeration of
Probiotic Bacteria
Flow VIT® combines Fluorescence in Situ Hybridization (FISH) with flow cytometry.
This powerful method enables the rapid and specific enumeration of
probiotic microorganisms in complex microbial communities and ensures that only
living cells are specifically identified and quantified.
Breakthrough in the quality control of probiotics
The development of probiotics requires a deep microbiological understanding of the relationships and interactions between the individual microorganisms as well as with the microbiological ecosystem of the host. In addition, the production and quality control of probiotics requires precise cell count and activity determination of the individual bacterial species in probiotic mixtures, including non-cultivable bacteria.
However, conventional (cultivation) and novel microbiological methods (Next Generation Sequencing (NGS) and flow cytometry) face obstacles and limitations that must be overcome to gain the necessary insights.
Flow VIT® technology overcomes these limitations and enables the direct and accurate quantification of viable cells - quickly, safely and specifically.
Implementation
The implementation of the Flow VIT® technology is user-friendly and robust. The test kits are easy to use, require no special prior knowledge for integration into routine laboratory workflows, and are compatible with standard flow cytometers. The automated workflow reduces manual effort and minimizes sources of error.
The following standard Flow VIT® test kits are now available:
Flow VIT® Lactobacillus acidophilus
Flow VIT® Lactobacillus rhamnosus
Flow VIT® Lactobacillus plantarum
Flow VIT® Bifidobacterium breve
Flow VIT® Bifidobacterium longum
Flow VIT® Bifidobacterium lactis
Flow VIT® Bifidobacterium spp.
Are you looking for a test kit for the rapid enumeration of a different species? We continuously expand our portfolio of standard test kits and offer customized test kits for single- or multi-species mixtures. For more information, please contact us!
Consulting and services
For probiotic products and microbiome analyses, we also offer a comprehensive range of laboratory and service solutions. Our standard services cover a wide range of analyses relevant to the entire probiotic production process – from research and development to quality control of the final product. In addition to the proven Flow VIT® method and VIT® technology, we utilize flow cytometry, RT-qPCR, dPCR, 16S rDNA sequencing, and next-generation sequencing, complemented by customized solutions for precise analyses.
Microbiome analytics is a highly sensitive field. To ensure the highest level of security for our customers, our services are certified according to GMP (Good Manufacturing Practice) and ISO 9001:2015 standards.
Rapid and highly specific enumeration of multiple bacterial species in the same sample
Viability and activity analysis
Validation of stability and viability throughout the entire shelf life
Validation of the composition of final products
Monitoring of microbial quality
Control of contaminations
Spatial analysis, identification, and visualization
If you need further support or have any questions about our services and detection methods, please do not hesitate to contact our team of experts. We work in close cooperation with our customers. If an individual solution is required, we are happy to provide consultation.
Why vermicon?
vermicon supports pharmaceutical companies, CDMOs and developers of probiotic products with customized, alternative microbiological methods. Our direct approach enables the identification and visualization of bacteria directly in their natural environment and uncovers mechanisms and correlations that cannot be detected with pure sequence data or conventional cultivation methods. This enables us to deliver meaningful results quickly and efficiently.
Cutting-Edge Technologies
Flow VIT® integrates fluorescence in situ hybridization (FISH) with flow cytometry for rapid, specific, and accurate microbial analysis. This technology ensures the detection of only living cells and enables multi-species identification and enumeration in mixed cultures.
Versatile Applications
Our technologies are used in the probiotic, pharmaceutical and clinical research sectors, as well as in the water, wastewater and beverage industries. They provide valuable insights and enable reliable quality control. Our advanced solutions can accurately validate the viability, efficacy and stability of products.
Trusted Expertise
With GMP and ISO 9001:2015 certifications, more than 25 years of experience and a strong commitment to innovation, vermicon AG stands for reliable expertise in microbiome research. Our team of experts supports the entire process from development to market launch.