Detection of Enterococci
Rapid and specific identification of enterococci in water samples. VIT® Enteroccocus clearly detects all bacteria of the genus Enterococcus and in parallel Enterococcus faecalis in one analysis.
Enterococci are robust bacteria that can survive in the environment for a long time and are used as important indicators of fecal contamination. E. faecalis in particular is crucial for the assessment of microbiological water quality.
Your Advantages
VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.
The VIT® gene probes hybridize only with intact, metabolically active cells, as only these have sufficient amounts of ribosomal RNA. This means that only living Enterococcus and Enterococcus faecalis are detected, while dead or inactive cells are excluded.
Complete detection is completed in just 3-5 hours - a decisive advantage over cultivation-based methods, which take several days due to enrichment, incubation and evaluation. The VIT® technology therefore enables significantly faster decisions in quality assurance, process control and release.
The test kits are user-friendly and can be integrated into existing laboratory procedures with minimal training. Detection is carried out using fluorescence microscopy and does not require complex equipment or special software solutions. This makes the method particularly robust and user-independent.
The VIT® technology is based on over 25 years of experience in the development of specific, fluorescence-labeled gene probes for microbiological analysis. It is characterized by high specificity, reproducibility and resistance to interfering matrices.
A particular advantage: as the method is based directly on rRNA and does not require enzymatic amplification steps, it is insensitive to inhibitors. False-positive results, which can occur with PCR-based test kits - especially in complex matrices - are therefore ruled out.
Product Specifications
| Detection of | Enterococcus and Enterococcus faecalis |
| Samples that can be examined include | Isolates Enrichments |
| The technology | VIT® gene probe technology |
| Analysis | by means of VIT® adapted Fluorescence microscope |
| Type of analysis | qualitative detection |
| Scope of delivery | Analysis reagents Product manual |
| Package size | 25 analyses |
Handling and Analysis
The sample is prepared using a VIT® adapted fluorescence miscroscope. All bacteria are visible in phase contrast. Bacteria of the genus Enterococcus (without E. faecalis) glow green under fluorescent light excitation, Enterococcus faecalis glows red.
Would you like to know exactly which microorganisms are contained in your sample? Get to know our MIS (microbial identification service), e.g. for
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