Detection of Selected Antibiotic Resistance Genes in Wastewater
The qPCR VIT® AMR II Test Kit enables the specific and robust detection and quantification of the antibiotic resistance genes sul1 and blaNDM in treated wastewater samples using quantitative PCR (qPCR). The standardized analysis enables reliable and reproducible quantification of the selected target genes.
The blaNDM gene encodes a metallo-β-lactamase that can confer resistance to carbapenems. Carbapenems are among the most important reserve antibiotics in human medicine. The sul1 resistance gene confers sulfonamide resistance and is frequently used as a marker for antibiotic resistance genes in wastewater and environmental samples.
The qPCR Prep AMR Testkit provides the basis for sample preparation and is combined with the qPCR VIT® AMR I and qPCR VIT® AMR II Testkits for the molecular biological detection of selected antibiotic resistance genes. Together, the three Testkits form a coordinated analytical system that enables the detection of a broad spectrum of relevant antibiotic resistance genes in wastewater samples.
The vermicon AMR workflows are also used as part of the German nationwide AMELAG project by the Robert Koch Institute (RKI) and the German Federal Environment Agency (UBA) to analyze antibiotic resistance genes in complex wastewater samples.
Complete System for Detecting Antibiotic Resistance Genes in Wastewater
qPCR Prep AMR
Purification of wastewater samples for molecular biological qPCR analysis of antibiotic resistance genes
qPCR VIT® AMR I
Detection of β-lactam resistance genes:
- blaOXA-48-like,
- blaCTX-M, and
- blaKPC
qPCR VIT® AMR II
Detection of the β-lactam resistance gene and the sulfonamide resistance gene:
- blaNDM
- sul1
Your Benefits
Specially developed by experts in wastewater analysis. The optimized workflow ensures reliable detection of antibiotic resistance genes, even in complex wastewater samples with varied compositions.
Using a multiplex approach, it simultaneously detects the β-lactam resistance gene blaNDM and the sulfonamide resistance gene sul1. This makes it possible to detect a carbapenemase gene and a sulfonamide resistance gene simultaneously in a single test run.
The two resistance genes are detected and quantified in parallel using a multiplex qPCR assay. The extraction control (DNA spike-in) included in the kit allows for verification of the efficiency of sample purification.
The AMR workflows developed by vermicon AG are being used as part of the AMELAG project for the molecular biological qPCR analysis of antibiotic resistance genes in wastewater. The findings obtained through this work are continuously incorporated into the optimization and further development of the analytical methods.
The qPCR VIT® product line is a comprehensive system for wastewater surveillance. It combines sample preparation, analysis, evaluation, and monitoring of health-related viruses and antibiotic resistance genes - to provide scientifically sound, comparable, and reliable data.
Directive (EU) 2024/3019 mandates the monitoring of antimicrobial resistance in municipal wastewater. The qPCR VIT® AMR product line enables the standardized detection of selected antibiotic resistance genes, thereby supporting the implementation of such AMR monitoring.
Product Specifications
| Detection | Molecular qPCR-based detection of the β-lactam resistance gene blaNDM and the sulfonamide resistance gene sul1 in wastewater samples |
| Detection control | Positive and negative controls |
| Extraction control (DNA Spike-In) to verify the efficiency of sample purification | |
| Result | Quantitative detection of antibiotic resistance genes using qPCR analysis |
| Results reported in, for example, gene copies/L using quantification standards included in the kit | |
| Contents | All essential consumables are included in the test kit |
| Packaging Size | 100 reactions per test kit |
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