Detection of Pathogenic Germs
Pseudomonas aeruginosa is a gram-negative bacterium that is widespread in the environment and is feared as a pathogen. Particularly in the beverage production industry, contamination of bottle washing systems or spray water by the bacterium can indicate contamination with surface water. The detection of the bacterium is of great importance for a company. The VIT® Pseudomonas aeruginosa detection system identifies Pseudomonas aeruginosa quickly and specifically. Only living bacteria are detected. The evaluation of the very simple analysis is clear and is carried out using a VIT® adapted fluorescence microscope.
Your Advantages
VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.
The VIT® gene probes hybridize only with intact, metabolically active cells, as only these have sufficient amounts of ribosomal RNA. This means that only living Pseudomonas aeruginosa are detected, while dead or inactive cells are excluded.
Complete detection is completed in just 3-5 hours - a decisive advantage over cultivation-based methods, which take several days due to enrichment, incubation and evaluation. The VIT® technology therefore enables significantly faster decisions in quality assurance, process control and release.
The test kits are user-friendly and can be integrated into existing laboratory procedures with minimal training. Detection is carried out using fluorescence microscopy and does not require complex equipment or special software solutions. This makes the method particularly robust and user-independent.
The VIT® technology is based on over 25 years of experience in the development of specific, fluorescence-labeled gene probes for microbiological analysis. It is characterized by high specificity, reproducibility and resistance to interfering matrices.
A particular advantage: as the method is based directly on rRNA and does not require enzymatic amplification steps, it is insensitive to inhibitors. False-positive results, which can occur with PCR-based test kits - especially in complex matrices - are therefore ruled out.
Product Specifications
| Detection of | Pseudomonas aeruginosa |
| Samples that can be examined include | Isolates (direct) Liquid enrichment (direct) Rinse water (short pre-enrichment) |
| Technology | VIT® gene probe technology |
| Evaluation | by means of VIT® adapted Fluorescence microscope |
| Type of analysis | qualitative detection |
| Scope of delivery | Analysis reagents Product manual |
| Package size | 25 analyses |
Handling and Analysis
The sample is prepared using a VIT® adapted fluorescence miscroscope. All bacteria are visible in phase contrast. All living cells glow red and Pseudomonas aeruginosa specifically green.
You can also have your sample analyzed in-house:
Laboratory analysis Pseudomonas aeruginosa
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