Laboratory analysis: Detection of Nitrotoga
The laboratory analysis VIT® Nitrotoga enables the identification and quantification of nitrite oxidizers of the genus Nitrotoga. This bacterial population is characterized by its particular resilience to low temperatures and extreme conditions. Nitrotoga is detected directly in the wastewater sample and analyzed quantitatively.
The laboratory analysis is ideal for monitoring nitrification processes. As Nitrotoga is stable even under difficult conditions, early detection provides valuable information for preventing malfunctions and optimizing process control. You can find out more about the advantages of Nitrotoga in this article.
The report contains an informative photo documentation that visualizes Nitrotoga bacteria specifically in the sample.
Qualitative or quantitative detection?
Quantitative detection
How is the analysis performed?
Using the reliable VIT® gene probe technology.
What are the requirements for the sample to be sent in?
Please request our simple fixation protocol for sampling wastewater samples and fix/stabilize your sample according to this procedure prior to shipment.
Our test kit is ideal for a quick analysis at your site:
Your Advantages
VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.
The technology enables direct quantification of the target organisms in the sample. Using the VIT® Vision software, the population development can be recorded and reliably tracked over time. Even slight changes in the density of AOB and NOB become visible even before the first abnormalities in the process parameters become apparent. This enables proactive intervention - for example, if the nitrite oxidizers begin to decline, even before nitrite accumulates in the process.
The analysis is carried out without complex sample purification or enrichment: the microorganisms are hybridized directly in activated sludge or other environmental samples and then evaluated microscopically. This preserves the natural matrix - a major advantage over methods based on cell isolation or DNA extraction.
The VIT® gene probes hybridize only with intact, metabolically active cells, as only these have sufficient amounts of ribosomal RNA. This means that only living nitrite oxidizers of the genus Nitrotoga are detected, while dead or inactive cells are excluded.
In contrast, PCR only detects DNA, regardless of whether the cell is alive or dead. This means that dead cells can also be detected in PCR analyses, which can lead to a considerable overestimation of the actual biological activity - particularly problematic in processes such as nitrification, where the functional potential is crucial.
Depending on the setup, it only takes a few hours from sample extraction to analysis. The hybridization is standardized, the analysis is performed by fluorescence microscopy and can also be carried out on site in routine environments. The evaluation and subsequent quantification can be carried out easily with the help of the VIT® Vision software.
The test kits are user-friendly and can be integrated into existing laboratory procedures with minimal training. Detection is carried out using fluorescence microscopy and does not require complex equipment or special software solutions. This makes the method particularly robust and user-independent.
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