Detection of Cronobacter in food samples
The detection of Cronobacter spp. (formerly: Enterobacter sakazakii) in particular places the highest demands on the precision and reliability of detection methods. With VIT® Cronobacter, all bacterial species of the genus Cronobacter are detected with reassuring certainty. And with astonishing speed.
The detection is easy to carry out - it only takes a few minutes. The results of the analysis are available after just 3 hours. The analysis is carried out using a fluorescence microscope. All essential consumables for carrying out the analysis are included in the kit. Package size: 25 analyses.
Your advantages
VIT® technology enables the targeted detection of individual microorganisms at population, genus or species level. The use of highly specific, rRNA-based gene probes ensures clear identification of the target organism directly in the sample.
The VIT® gene probes hybridize only with intact, metabolically active cells, as only these have sufficient amounts of ribosomal RNA. This means that only living Cronobacter are detected, while dead or inactive cells are excluded.
Complete detection is completed in just 3-5 hours - a decisive advantage over cultivation-based methods, which take several days due to enrichment, incubation and evaluation. The VIT® technology therefore enables significantly faster decisions in quality assurance, process control and release.
The test kits are user-friendly and can be integrated into existing laboratory procedures with minimal training. Detection is carried out using fluorescence microscopy and does not require complex equipment or special software solutions. This makes the method particularly robust and user-independent.
The VIT® technology is based on over 25 years of experience in the development of specific, fluorescence-labeled gene probes for microbiological analysis. It is characterized by high specificity, reproducibility and resistance to interfering matrices.
A particular advantage: as the method is based directly on rRNA and does not require enzymatic amplification steps, it is insensitive to inhibitors. False-positive results, which can occur with PCR-based test kits - especially in complex matrices - are therefore ruled out.
Product specifications
| Detection of | Cronobacter |
| Samples that can be examined include | Isolates (direct) Liquid enrichment (direct) Water (short pre-enrichment) Food (short pre-enrichment) Milk powder (short pre-enrichment) Hygiene samples (short pre-enrichment) |
| Technology | VIT® gene probe technology |
| Evaluation | by means of VIT® adapted Fluorescence microscope |
| Type of analysis | qualitative detection |
| Scope of delivery | Analysis reagents Product manual |
| Package size | 25 analyses |
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