Summary
This study presents an optimized method for combining flow cytometry and fluorescence in situ hybridization (Flow-FISH) to precisely quantify Gram-positive bacteria in probiotics. This method overcomes the limitations of conventional methods by combining the speed and automation of flow cytometry with the high specificity of the FISH technique. This allows viable cells to be distinguished at species level in probiotic mixtures.
Lyophilized samples of Lacticaseibacillus rhamnosus, Lactiplantibacillus plantarum, Bifidobacterium animalis subsp. lactis and a commercial product were investigated. The optimized Flow-FISH protocol is characterized by shortened hybridization times (1.5 hours) and the elimination of centrifugation steps.
Quote
Snaidr, L., Mühlhahn, P., Beimfohr, C., Kreuzer, C., Richly, C., & Snaidr, J. (2024). Specific cultivation-independent enumeration of viable cells in probiotic products using a combination of fluorescence in situ hybridization and flow cytometry. Frontiers in Microbiology, 15, Article 1410709. https://doi.org/10.3389/fmicb.2024.1410709