Monitoring of Bulking Sludg
Molecular monitoring of bulking sludge in industrial wastewater treatment plants
Abstract
Fluorescence In Situ Hybridisation (FISH) adapted for the industrial microbiology in the form of VIT® (vermicon identification technology) was used to monitor the presence of filamentous microorganisms in industrial wastewater treatment plants (WWTPs). Monitoring with restricted set of VIT® probes in WWTPs from potato industry showed growth and decline of Thiothrix populations that could be linked to operational procedures. In a follow up project new VIT® probes were developed for filamentous bacteria in industrial WWTPs and 70 WWTPs were analyzed for presence of these filaments. Several newly described species of filamentous bacteria appear to be common and dominant in industrial WWTPs. Monitoring of a WWTP from textile industry showed growth and decline of one of these organisms when operational conditions in the plant were varied. The present paper demonstrates that bulking sludge in industrial wastewater treatment plants can effectively be monitored using a combination of standard chemical analysis and the VIT® technology.
Publikation language: English
Identical microscopic image after VIT® analysis: phase contrast, all viable Thiothrix and Eikelboom 021 filaments shine red, 021 filaments shine additionally specifically green.
Quotation
J. van der Waarde, J. Krooneman, B. Geurkink, A. van der Werf, D. Eikelboom, C. Beimfohr, J. Snaidr, C. Levantesi, V. Tandoi (2002): Molecular monitoring of bulking sludge in industrial wastewater treatment plants. Water Sci Technol. 2002;46(1-2), p. 551-558.